• To better characterize the Carnegie stage primate PGCs

  2018-10-22

  

  To better characterize the Carnegie stage 23 primate PGCs, we performed immunofluorescence for the basement membrane protein Laminin and the PGC marker OCT4 (Figure 2A). All OCT4-positive PGCs were located within the Laminin-positive cords in the testicular cortex at this age. We also discovered tha

• br Experimental Procedures br Author Contributions br Acknow

  2018-10-22

  

  Experimental Procedures Author Contributions Acknowledgments This work was supported in part by the Novel Bioengineering and Technological Approaches to Solve Two Major Health Problems in Taiwan sponsored by the Taiwan Ministry of Science and Technology Academic Excellence Program under gra

• So far literature on A R has been in

  2018-10-22

  

  So far, literature on A1R has been in favor of a prevailing role played by these receptors in the activation of osteoclasts (Kara et al., 2010; He and Cronstein, 2012) and some findings showed that the blockade of these AR may induce favorable effects on osteoporosis or fracture healing. However, ou

• M macrophage derived factors such as FGF

  2018-10-20

  

  M2 macrophage-derived factors, such as FGF2, IGF-1, PDGF, TGFβ1 and VEGF are angiogenic, limiting cell death due to lack of blood supply (Nakao-Hayashi et al., 1992; Nahrendorf et al., 2007; Grant et al., 1993b; Roberts et al., 1986). Angiogenesis is also supported by M2 macrophage production of ECM

• domoic acid Although both c Kit Lin and Sca Lin have been

  2018-10-20

  

  Although both c-Kit+Lin− and Sca-1+Lin− have been shown to be undifferentiated cardiac cells with differentiation ability, a clear difference seems to have been established between these cells at the molecular level (Dey et al., 2013). A study of their transcriptional profiles revealed that c-Kit+ r

• The PBMC iPS F cell line has been adapted to

  2018-10-20

  

  The PBMC1-iPS4F1 cell line has been adapted to feeder-free culture conditions and displays a normal karyotype (46, XX) after extensive mechanical and enzymatic transfers (Fig. 1D). The iPSC line was also characterized using a genetic fingerprinting assay by microsatellite analysis. The endogenous e

• Runx also controls the proliferation and neuronal differenti

  2018-10-20

  

  Runx1 also controls the proliferation and neuronal differentiation of certain neural progenitor cell populations. In embryonic olfactory bulb progenitor cells in vivo, as well as in cultures of either embryonic olfactory progenitor cells or embryonic cortical NSPCs, Runx1 increases cell proliferatio

• br Resource details To generate the human

  2018-10-20

  

  Resource details To generate the human iPSC line, N44SV.5, non-integrative Sendai viruses containing the reprogramming factors Oct3/4, Sox2, cMyc, Klf4 have been employed (Takahashi et al., 2007). For this purpose, commercially available fibroblasts from a healthy juvenile donor were acquired in

• br In vitro differentiation Media was removed

  2018-10-20

  

  In vitro differentiation Media was removed and the cells were washed with PBS. TrypLE was added for 3min. DMEM containing 20% FCS was added to quench the TrypLE. Cells were centrifuged at 300×g for 3min and the cell pellet was resuspended in APEL medium (Stemcell Technologies) containing 10μM Roc

• Here we demonstrate that the

  2018-10-20

  

  Here, we demonstrate that the methylation patterns of the H19/IGF2 and SNRPN DMRs were stable from the early stages of derivation to late passages of monkey ESCs, and following in vitro differentiation. We also showed that the H19/IGF2 DMR methylation pattern was correlated with the capacity of earl

• br Materials and method br

  2018-10-20

  

  Materials and method Results Discussion Here we took advantage of a well-characterized model of skeletal muscle damage to study the effect of FAP ablation on skeletal muscle regeneration in vivo. Our results show that Nilotinib can effectively prevent transient ECM deposition following acut

• br Results A total of iNSCs were stereotactically transplant

  2018-10-20

  

  Results A total of 2.25 × 105 iNSCs were stereotactically transplanted into the cortex and hilus of the dentate gyrus of adult female immunodeficient mice (8 weeks; ∼25 g; n = 9; Figure 1A). We chose the cortex as one target site because this region has a well-described tissue architecture, inclu

• Whole exome sequencing of tumor

  2018-10-20

  

  Whole-exome sequencing of tumor DNA from GBM 5 identified a total of 32 SNVs (Table S3). A subset of these SNVs was selected for single-cell analysis based on putative gene function and variant allele fractions encompassing high, low, and intermediate frequencies. The genomic targets selected includ

• Our data confirm previously unrecognized levels of temporal

  2018-10-20

  

  Our data confirm previously unrecognized levels of temporal diversity and complexity in the subclonal landscape of GBM. In all cases analyzed, genetically distinct subclones had variable serial repopulating activity in vivo. We can exclude the possibility that the presence of in vitro aberrations pl

• Recently several clinical trials of stem cell

  2018-10-20

  

  Recently, several clinical trials of stem-cell-based therapy for SCI using either human NS/PCs (Cummings et al., 2005; Salazar et al., 2010) or human ESC-derived OPCs (Strauss, 2010) have been initiated. Compared with these stem cells, iPSCs raise fewer ethical concerns in certain countries (Nori et

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