• domoic acid Although both c Kit Lin and Sca Lin have been

  2018-10-20

  

  Although both c-Kit+Lin− and Sca-1+Lin− have been shown to be undifferentiated cardiac cells with differentiation ability, a clear difference seems to have been established between these cells at the molecular level (Dey et al., 2013). A study of their transcriptional profiles revealed that c-Kit+ r

• The PBMC iPS F cell line has been adapted to

  2018-10-20

  

  The PBMC1-iPS4F1 cell line has been adapted to feeder-free culture conditions and displays a normal karyotype (46, XX) after extensive mechanical and enzymatic transfers (Fig. 1D). The iPSC line was also characterized using a genetic fingerprinting assay by microsatellite analysis. The endogenous e

• Runx also controls the proliferation and neuronal differenti

  2018-10-20

  

  Runx1 also controls the proliferation and neuronal differentiation of certain neural progenitor cell populations. In embryonic olfactory bulb progenitor cells in vivo, as well as in cultures of either embryonic olfactory progenitor cells or embryonic cortical NSPCs, Runx1 increases cell proliferatio

• br Resource details To generate the human

  2018-10-20

  

  Resource details To generate the human iPSC line, N44SV.5, non-integrative Sendai viruses containing the reprogramming factors Oct3/4, Sox2, cMyc, Klf4 have been employed (Takahashi et al., 2007). For this purpose, commercially available fibroblasts from a healthy juvenile donor were acquired in

• br In vitro differentiation Media was removed

  2018-10-20

  

  In vitro differentiation Media was removed and the cells were washed with PBS. TrypLE was added for 3min. DMEM containing 20% FCS was added to quench the TrypLE. Cells were centrifuged at 300×g for 3min and the cell pellet was resuspended in APEL medium (Stemcell Technologies) containing 10μM Roc

• Here we demonstrate that the

  2018-10-20

  

  Here, we demonstrate that the methylation patterns of the H19/IGF2 and SNRPN DMRs were stable from the early stages of derivation to late passages of monkey ESCs, and following in vitro differentiation. We also showed that the H19/IGF2 DMR methylation pattern was correlated with the capacity of earl

• br Materials and method br

  2018-10-20

  

  Materials and method Results Discussion Here we took advantage of a well-characterized model of skeletal muscle damage to study the effect of FAP ablation on skeletal muscle regeneration in vivo. Our results show that Nilotinib can effectively prevent transient ECM deposition following acut

• br Results A total of iNSCs were stereotactically transplant

  2018-10-20

  

  Results A total of 2.25 × 105 iNSCs were stereotactically transplanted into the cortex and hilus of the dentate gyrus of adult female immunodeficient mice (8 weeks; ∼25 g; n = 9; Figure 1A). We chose the cortex as one target site because this region has a well-described tissue architecture, inclu

• Whole exome sequencing of tumor

  2018-10-20

  

  Whole-exome sequencing of tumor DNA from GBM 5 identified a total of 32 SNVs (Table S3). A subset of these SNVs was selected for single-cell analysis based on putative gene function and variant allele fractions encompassing high, low, and intermediate frequencies. The genomic targets selected includ

• Our data confirm previously unrecognized levels of temporal

  2018-10-20

  

  Our data confirm previously unrecognized levels of temporal diversity and complexity in the subclonal landscape of GBM. In all cases analyzed, genetically distinct subclones had variable serial repopulating activity in vivo. We can exclude the possibility that the presence of in vitro aberrations pl

• Recently several clinical trials of stem cell

  2018-10-20

  

  Recently, several clinical trials of stem-cell-based therapy for SCI using either human NS/PCs (Cummings et al., 2005; Salazar et al., 2010) or human ESC-derived OPCs (Strauss, 2010) have been initiated. Compared with these stem cells, iPSCs raise fewer ethical concerns in certain countries (Nori et

• br Funding This work was funded by NIHR GM

  2018-10-20

  

  Funding This work was funded by NIHR01GM085456 awarded to Tanja Dominko. Declaration of interest The following are the supplementary data related to this article. Acknowledgements Introduction The identification of culture conditions that promote the expansion of primitive hematopoieti

• br Introduction Visional signals are

  2018-10-20

  

  Introduction Visional signals are mediated by photoreceptors, bipolar Radicicol and ganglion cells, and the loss of these neurons leads to irreversible blindness (de Jong, 2006; Friedman et al., 2004; Hartong et al., 2006; Quigley and Broman, 2006; Ramsden et al., 2013; Thylefors et al., 1995). P

• Next we unraveled a mechanism by

  2018-10-20

  

  Next, we unraveled a mechanism by which exogenous ANXA3 proteins are internalized in HCC GSK1070916 and characterized the mechanism by which ANXA3 promotes CSC-like properties following its internalization. There has been no study regarding the route of entry of exogenous ANXA3 in any cell type thus

• It is interesting that OB

  2018-10-20

  

  It is interesting that OB neurons produced from the transient Gli1-enriched subcallosal domain in juvenile mice mostly corresponded to sGCs, when they were derived from a region with active SHH signaling. It is possible that the relative levels of SHH signaling are adjusted in different domains. The

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